MS-HRM to Detect Serum DNA Methylation of Intrauterine Growth Retardation Children
Intrauterine growth retardation (IUGR) is also called fetal growth restriction (FGR),which is the major complications in obstetrics and one of the most important causation from high morbidity and mortality in perinatal.In this study,MS-HRM (Methylation-Sensitive High Resolution Melting Curve Analysis) was used to detect the methylation status of serum DNA.Gene of insulin-like growth factor binding protein 3 (IGFBP-3) was detected in this study.Results showed that the serum DNA methylation level of FGR fetus were lower than that of the control group; different methylation levels were also found between male and female fetus; and the methylation level was increased with the birth weight of the newborn.Our results showed that the IGFBP-3 gene methylation level of serum DNA of newborn could be semi-quantitative detected which guide the early prevention and treatment of IUGR.It also indicated that the methylation status of serum DNA can be conveniently identified and quantified by inspection of the melting curves.
MS-HRM IUGR IGFBP-3 Serum DNA
Yan Du Youxia Zhou Qian Wu
Department of Pathology, Maternity and Childrens Health Care Centers in Wuxi, Wuxi, China,;Research Research Center of Learning Science, Southeast University,Nanjing, China State Key Laboratory of Reproductive Medicine,Department of Hygienic Analysis and Detection, School
国际会议
北京
英文
106-109
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）