Leptin Receptor in Ram Epididymal Spermatozoa

摘要：

　　This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR.Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed.To purify spermatozoa,motile sperm were isolated by the swim-up procedure.Total RNA was isolated from epididymal spermatozoa and placental cotyledon,as a positive control,and then they were purified.Specific bands (98 and 308 bp)for Ob-R and Ob-Rb were detected after RT-PCR in both epididymal spermatozoa and placental cotyledon.We may conclude that Ob-R and Ob-Rb mRNA are present in ram epididymal spermatozoa and leptin perhaps exerts physiological effects,as already demonstrated in human.

关键词： Epididymal spermatozoa rnRNA Ob-R Ob-Rb

作者: Mohammad Roostaei-Ali Mehr Reza Rajabi-Toustani Rasool Motamedi-Mojdehi

作者单位: Department of Animal Science Faculty of Agricultural Sciences, University of Guilan Rasht, Iran

会议类型: 国际会议

会议名称: 2012世界工程和技术大会

会议地点: 北京

会议语种:英文

页码: 121-123

在线出版日期: 2012-10-26（万方平台首次上网日期，不代表论文的发表时间）