Engineering Antibody Fragment with the Quantum Dot in Cancer Cell Imaging and Diagnosis
The conjugates of monoclonal antibodies and nanoparticles,including quantum-dot(QD),offer significant advantages over conventional fluorescent probes to image and study biological processes.The extend stability,intense fluorescence and low toxicity of QDs are well suited for biological applications.In the present study,we used QD-conjugated anti-glucose-regulated protein 78(GRP78)antibody to examine the gene expression in prostate cancer ceils under the unfolded protein response (UPR).QDs have got unique,simple and fast properties over current diagnostic techniques such as peroxidase-based immunohistochemical staining procedures,therefore the nanocarrier-conjugated antibody fragment has potential to become a new therapeutic tool for cancer diagnosis and treatment.
Quantum Dot Antibody Fragment Prostate Cancer GRP78 scFv UPR Immunohistochemistry
Lizhi Liu Weiming Xu
Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield Western Bank, Firth Court, Sheffield, UK S10 2TN
国际会议
北京
英文
126-128
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）