Functional analysis of OAT gene in Aspergillus niger
Mitochondrial oxaloacetate transporter protein encoded by OAT gene transports oxaloacetate from cytoplasm into mitochondria.To investigate the primary effects of OAT gene on relative metabolism in Aspergillus niger,a oat-deleted mutant was derived from wild-type A.niger ATCC 1015 using the double-crossover chromosome replacement technique.Then batch fermentation was performed to evaluate the mutant.Compared with the wild type,the mutant showed lower organic acids production,with the experimental data that the production of pyruvate and 2-ketoglutarate decreased by 31.6% and 35.7%,respectively,and by contrast,the mutant showed higher glycerol formation.The results suggest that OAT gene plays significant roles on metabolism in A.niger.
Aspergillus niger oxaloacetate transporter organic acids glycerol formation
Chuang Zhou Xihong He Hao Liu Hao Liu
MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin Univer College of B iotechnology, Tianjin University of Science & Technology, Tianjin, China
国际会议
北京
英文
139-141
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）