The mechanism of lipofectamine 2000 mediated transmembrane gene delivery

摘要：

　　In this paper,the relatived mechanism between lipofectamine 2000 mediated transmembrane gene delivery and endocytic pathway were investigated.Clathrin and caveolae-mediated endocytic pathway contributions to transfection efficiency were studied.The inhibitors of endocytosis were used to treat HEp-2 cells before lipofectamine 2000/pGFP-N2 transfection.Transfection efficiency was evaluated with green fluorescence protein (GFP) expression assays.Cell viability and cytotoxicity were evaluated with MTT method.The results indicated that inhibitors of clathrin (chlorpromazine or wortmannin) and caveolin (genistein) could reduce the cell transfection efficiency observably.Both clathrin and caveolae-mediated endocytic pathways play important roles in transmembrane gene delivery.

关键词： Gene delivery Cationic liposomes Transfection efficiency Endocytic pathway Inhibitor

作者: Shaohui Cui Shubiao Zhang Huiying Chen Bing Wang Yinan Zhao Defu Zhi

作者单位: Key Laboratory of Bio-chemistry Engineering-The State Ethnic Affairs Commission-Ministry of Education Dalian Nationalities University Dalian, China

会议类型: 国际会议

会议名称: 2012世界工程和技术大会

会议地点: 北京

会议语种:英文

页码: 172-175

在线出版日期: 2012-10-26（万方平台首次上网日期，不代表论文的发表时间）