Preparation of water soluble yeast glucan by four kinds of solubilizing processes
(1→3)-β-D-glucan from the inner cell wall of Saccharomyces cerevisiae is considered a member of a class of drugs known as biological response modifiers (BRM).However the glucan was an insoluble polysaccharide,which could be the major barrier to the utilization of glucan.In this case,the insoluble glucan was convent into a soluble form by four kind of solubilizing processes.The yield,solubility,chemistry structure and immunoprophylaxis efficacy of the soluble products were compared.Our date suggest that urea has a significant effect on yield,and DMSO has a significant effect on solubility.FT-IR spectra,13C NMR spectra and helix-coil transition analysis demonstrate that the chemistry structure of native and solubilizing glucans have no significant difference.They still have the triple helical structure.The solubility and immunoprophylaxis efficacy assay indicate that the introduction of phosphate group not only enhanced the solubility of glucan,but also improved the survival rate of mice challenged with E.coli.
Glucan Solubility FT-IR spectra 13C NMR spectra Immunoprophylaxis efficacy
Liping Du Xuekuang Zhang ChaoWang Dongguang Xiao
Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Industrial Microbiology Ke Key Laboratory of lndustrial Microbiology, Ministry of Education, Tianjin Industrial Microbiology Ke
国际会议
北京
英文
184-188
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）