Determination of Chitosan Oligosaccharide Content by Acetylacetone Spectrophotometry Method

摘要：

　　Objective:To establish a common method to detect the content of chitosan oligosaccharide.Methods:Chitosan oligosaccharide was hydrolyzed completely by concentrated hydrochloric acid,and the solution was regulated into neutral with NaOH.Then,determined the absorbance in 525nm,and substituted into the regression equation to determine the results.Results:The results showed there was a good linear relationship when the concentration of chitosan oligosaccharide ranged from 0.02 mg/mL to 0.12 mg/mL,r2 =0.999.The average recovery of chitosan oligosaccharide samples was 99.25%.Conclusion:The method is sensitive,accurate and simple.It is applied to determine of the content of chitosan oligosaccharide.

关键词： Chitosan Oligosaccharide Acetylacetone Spectrophotometry method Content Determination

作者: Zeping Xu Chuanlun Yang Xinqing Zhang Xiuzhi Wang Baosheng Huang

作者单位: The Technological Centre of Shandong Chambroad Holding Co.,Ltd.Binzhou,256500,China

会议类型: 国际会议

会议名称: the 2012 2nd International Conference on Frontiers of Manufacturing Science and Measuring Technology (第二届制造科学与检测技术国际会议(ICFMM 2012))

会议地点: 西安

会议语种:英文

页码: 543-547

在线出版日期: 2012-06-12（万方平台首次上网日期，不代表论文的发表时间）