Chitosan metal complex:an artificial metalloprotease hydrolyzing Trypsin Inhibitor from Soybean
Most popular agents for protein cleavage are proteolytic enzymes,but they require more rigorous hydrolytic conditions,and it is very difficulty to separate them from the productions.In this paper,an new artificial metalloprotease,Cu(Ⅱ) complex of cyclen (Cu(Ⅱ)Cyc) using chitosan as supporter,was designed and synthesized successfully.The hydrolytic efficient under different conditions was measured and confirmed with HPLC,SDS-PAGE,further more.Some reaction conditions,such as pH,temperature,were researched,and the results show that the optimum reaction time was 48 h,temperature was 60 ℃ respectively,The observed rate constants for trypsase inhibitor cleavage was 1.006× 10-2 h-1 under above conditions,and the observed rate constants was 2.120× 10-2 h-1 when the pH was 9.0.This paper suggested that the chitosan metal complexes could efficiently accelerate the hydrolysis reaction.
Metal complex metalloprotease trypsase inhibitor Hydrolysis hydration
Peng Liang Dongfeng Wang Xingyu Wang Mei Ding Li Zhang Ying Xu
Food Chemical Laboratory, College of Food Science and Engineering, Ocean University of China, Qingda Food Molecular Biology Laboratory, College of Food Science and Engineering, Ocean University of Chin
国际会议
厦门
英文
414-418
2012-01-04（万方平台首次上网日期，不代表论文的发表时间）