Serum proteins opsonization and phagocytic uptake of PEG-modified PLGA nanoparticles:Effect of particle size
The purpose of this study is to evaluate the effect of particle size on serum protein opsonization and in vitro macrophage uptake of polyethyleneglycol modified poly (D,L-lactide-co-glycolide) nanoparticles (PEG-PLGA-NPs).PEG-PLGA-NPs were prepared by modified-spontaneous emulsification solvent diffusion (modified-SESD) method.Serum protein adsorptions to PEG-PLGA-NPs were evaluated by bicinchoninic acid (BCA) protein assay and enzyme-linked immunosorbent assay (ELISA).Complement activation was also investigated by ELISA for complement fragments iC3b.Uptake of PEG-PLGA-NPs by macrophages was measured by fluorescence spectrometer.The results showed that serum protein adsorption and complement activation were augmented for nanoparticles with a larger size below 400 nm.Phagocytosis of PEG-PLGA-NPs by murine peritoneal macrophages involved serum-independent and serum-dependent phagocytosis.Serum-independent phagocytosis decreased,while serum-dependent phagocytosis increased with the increase of particle size in the nanometer and submicrometer range.Consequently,nanoparticles with size of about 400 nm were phagocytosed more readily than either smaller or larger particles.
PEG-PLGA nanoparticles Particle size Serum proteins adsorption Complement activation Phagocytic uptake
Anshu Yang Wei Liu Xiangliang Yang
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047,China College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074,
国际会议
昆明
英文
939-942
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）