Study on Synthesis and Characterization of a Fluorescence Probe and Its Application for Determination of Superoxide Anion Radical
A novel Schiff base,2-(Salicylideneamino)pyridine (2-SAP) synthesized by 2-aminopyridine and salicylaldehyde was used as a fluorescence probe to determine superoxide anion radical (O2·-) in this study.The synthetic product was characterized by IR,1H-NMR and GC-MS methods.A fluorescence quenching reaction occurs between 2-SAP and O2·-,and the fluorescence intensity decreased significantly.The effect of pyrogallol concentration (2.0× 10-5M to l.0× 10-3 M) was discussed in this study.In the range from 2.0×10-5 M to 1.0×l0-4 M,relative fluorescence intensity (y) and pyrogallol concentration (x) shown a good linear relationship,y=37.801x+76.854,r=0.993.Based on this phenomenon,the O2·-generated by pyrogallol autoxidation could be determined simply with high sensitivity.
synthesis characterization fluorescence probe superoxide anion radical
Yongqiang Zhao Hong Lin Laihao Li Xiangqing Yang Shuxian Hao Jianwei Cen Ya Wei Bo Qi Hui Huang
Food Safety Laboratory, Ocean University of China, Qingdao, China;National R&D Center for Aquatic Pr Food Safety Laboratory, Ocean University of China, Qingdao, China National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Ch
国际会议
昆明
英文
1483-1487
2011-12-28（万方平台首次上网日期，不代表论文的发表时间）