Mechanical Characterization of Hepatoma Cells Using Atomic Force Microscope
(0) In order to reveal variation of mechanical properties of hepatoma cells with nanometer resolution,atomic force microscopy (AFM)-based nanoindentation experiments are performed on hepatoma cell to derive Youngs modulus employing a corrected Hertz model.Under load conditions of nanoindentation force as 0.43809-0.73015nN and penetration rate as 0.4 Hz,the calculated value of Youngs modulus of hepatoma cells is 34.137±0.67kPa with a 95% confidence interval.The results demonstrate the Youngs modulus varies with the measurement position,and the center of cell possesses lower value than peripheral region.Variation of Youngs modulus is resulted from external reaction,which supports well the theory of cytoskeleton structure.Furthermore,the difference of Youngs modulus between normal cells and cancerous ones are also discussed,and it will provide possibility of a new route for early diagnosis of hepatoma.
Atomic Force Microscope Hepatoma Cell Youngs Modulus
Jinghe Wang Miao YU Li Liu Jie zhao Hongxiang Wang
State Key Laboratory of Robotics and System,Harbin Institute of Technology,Heilongjiang Harbin,China Center for Precision Engineering,Harbin Institute of Technology,Heilongjiang Harbin,China 150001 Center for Precision Engineering,Harbin Institute of Technology,Heilongjiang Harbin,China 150001;The
国际会议
大理
英文
869-873
2011-08-18（万方平台首次上网日期，不代表论文的发表时间）