Delivery of miRNA using Fe2O3 Nanoparticles capped Polyethyleneimine as a nonviral carrier
An efficient and safe delivery system of RNA interfering is required for clinical application of gene therapy.The study aimed to develop Fe2O3-based nanoparticles for gene delivery to overcome the disadvantages of polyethyleneimine (PEI) or cationic liposome as gene carrier including the cytotoxicity caused by positive charge and aggregation in the cells surface.PEI-capped Fe2O3 nanoparticles are successfully manufactured utilizing Fe2O3 as core,PEI as carapace,which bind miRNA at an appropriate weight ratio by electrostatic interaction and result in well-dispersed nanoparticles.The synthesized GFP tag with miR-26a expression plasmid was used for monitoring transfection efficiency in HepG2 cells.The nanocomplex exhibited higher transfection efficiency and lower cytotoxicity in HepG2 cells than the PEI/DNA complex and commercially available liposome.The delivery resulted in a significantly upregulation of miR-26a in HepG2 cells.Our results offer an alternate delivery system for RNA interfering that can be used on any gene of interest.
Gene Therapy Fe2O3 Nanoparticles Polytehyleneimine Mirna
Gaofeng Liang Ping Li Wanjun Lei
School of Medical Technology and Engineering, Henan University of Science and Technology, Luo Yang 471003, Henan, China
国际会议
广州
英文
543-546
2012-11-16（万方平台首次上网日期，不代表论文的发表时间）