A Hybrid-structured Microfluidic Chip Developed for ATP Bioluminescence Detection

摘要：

　　A hybrid-structured microfluidic chip integrating optical fiber sensing technology and bioluminescence chemical reaction mechanism was developed for rapid and sensitive detection of adenosine 5-triphosphate (ATP).This microfluidic chip was fabricated with five distinct layers for the successive steps of sample-enzyme reagent mixing,bioluminescence chemical reaction and scatting light collection.The experimental results demonstrated that the provided hybrid-structure improved the sensitivity,response time,reagent consumption and other properties of chip.This improvement enables the microfluidic chip to attain a sensitive ATP detection in the range from 10-9 to 10-4 M with excellent linearity (R2 =0.9967),low detection error (CV < 15%),and tiny reagent consumption (< 20 μL).These achievements thus indicated that the chip developed in this study possess the advantages such as high sensitivity,quick-response and small reagent consumption.

关键词： Microfluidic chip Bioluminescence detection ATP Fiber optics Micro mixer Biosensor

作者: Wu Ming Zhang Zhen Yu Li Tao Chen Jian Jiang Yao Yang Lv Min Li Guang Li

作者单位: State Key Laboratory of Industrial Control Technology, Zhenjiang University, Hangzhou 310027,P.R.China

会议类型: 国际会议

会议名称: the 2012 International Conference on Materials Science and Nanotechnology (2012年材料科学与纳米技术国际会议(ICMSN 2012))

会议地点: 广州

会议语种:英文

页码: 563-569

在线出版日期: 2012-11-16（万方平台首次上网日期，不代表论文的发表时间）