The bioconversion of green energy material (bamboo) to bioethanol using the two-stage organosolv and alkali pretreatment
Bamboos ability to grow on nutrient poor soils,little requirement of silvicultural management,easy harvesting characteristics,vegetative propagation,fast growth and a host of other desirable characteristics make it a good candidate as an energy crop.Energy crops are cultivated solely for use as sources of energy through their conversion into alcohols.This study set out to determine the potential of moso bamboo be used in the two-stage organosolv and alkaline pretreatment the production of bioethanol.Moso bamboo contains 63.3% (w/w) holocellulose and can serve as a low-cost feedstock for bioethanol production.Organosolv pretreatment (2% w/w H2SO4 in 75%w/w ethanol,160℃ for 30 min) of bamboo was further delignified through pretreatment of sodium hydroxide (10% and 20% w/w) or Calcium hydroxide(10% w/w),which resulted in about 96.5%(NaOH) and 85.7%(Ca(OH)2) lignin removal.The enzymatic hydrolysis of delignified cellulosic bamboo substrate with celluolase (15 FPU/g glucan) and β-glucosidase(30 IU/g glucan)showed 80.9%~95.5% saccharification after 48 h incubation at 50℃ and pH 4.8.Fermentation of enzymatic hydrolysates with Saccharomyces cerevisiae resulted in about 85.9%~93.5% of the corresponding theoretical ethanol yield after 24 h.
Green energy material moso bamboo bioethanol two-stage pretreatment enzymatic hydrolysis fermentation
Zhiqiang Li Zehui Jiang Benhua Fei Xinge Liu Yan Yu
International Centre for Bamboo and Rattan, Beijing 100102
国际会议
上海
英文
87-87
2012-10-19（万方平台首次上网日期，不代表论文的发表时间）