Enzyme immobilized on magnetic Fe3O4/CRGO nanocomposites:preparation and characterization
We proposed a one-step hydrothermal procedure to form Fe3O4 nanospheres on chemically reduced graphene oxide (CRGO) surfaces,and used this nanocomposites as substrates for enzyme immobilization.The as-prepared Fe3O4/CRGO nanocomposites were characterized using scanning electron microscopy (SEM),X-ray powder diffraction (XRD),FT-IR and vibrating sample magnetometer.Fe3O4 microspheres are randomly distributed on graphene sheets,the average diameter of Fe3O4 microspheres is about 260 nm.We used Horseradish peroxidase (HRP) as a model enzyme to investigate the immobilization activity.The HRP loading was 23.3mg/g supports and retained 70% of the first use after ten cycles.The catalyzed capability of immobilized HRP was investigated and the immobilized HRP exhibited broader pH stability and excellent reusability.The results show that the Fe3O4/CRGO nanocomposites are appropriate for the immobilization of enzyme,and could have potential use in practical.
Chemically reduced graphene oxide (CRGO) Fe3O4 horseradish peroxidase (HRP) enzyme immobilization
Wu Xiaochen Zhang Yan Wu Congyu Wu Haixia
Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240,China
国际会议
上海
英文
131-131
2012-10-19（万方平台首次上网日期，不代表论文的发表时间）