Interaction of human fibroblasts with electrospun composites gelatin/PLLA, chitosan/PLLA and PLLA fibrous scaffolds
Highly porous ultrafine electrospun scaffolds,gelatin/PLLA and chitosan/PLLA were prepared by blending gelatin and PLLA,chitosan and PLLA respectively.This bilaminar texture is suitable for preparing a bilayer skin substitute.The characterization of gelatin/PLLA and chitosan/PLLA scaffolds were examined by scanning electron microscopy (SEM) and compared respectively.The biocompatibilities of the gelatin/PLLA and chitosan/PLLA scaffolds were assessed by attachment,proliferation and viability of cells on them.The human fibroblasts cell line WI-38 was inoculated on gelatin/PLLA and chitosan/PLLA scaffolds,pure PLLA scaffolds as control.Our study indicated that over 30% WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2h after seeding,while the attachment of the cells was only 15% on PLLA scaffolds.Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells.Cell growth on the gelatin/PLLA and chitosan/PLLA scaffolds showed dramatic improvement,indicating a much better biocompatibility in the blends contributed by gelatin and chitosan.MTT assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells.These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.
PLLA gelatin chitosan electrospinning fibroblast cell culture
Li Jianbo Han Jun Ren Jie
School of Material Science and Engineering, Tongji University, Shanghai 200092, China; Department of School of Material Science and Engineering, Tongji University, Shanghai 200092, China School of Material Science and Engineering, Tongji University, Shanghai 200092, China; Key laborator
国际会议
上海
英文
187-187
2012-10-19（万方平台首次上网日期，不代表论文的发表时间）