Isolation of Bacillus subtilis, PCBs-degrading Strain

摘要：

　　A polychlorinated biphenyls-degrading bacterium was isolated from soil near the waste transformer.The strain,named as WFl,was able to use biphenyl as sole carbon source for growth.It was classified as Bacillus subtilis based on 16S rRNA sequence analysis.The effects temperature,pH,initial polychlorinated biphenyls concentration on polychlorinated biphenyls degradation ability of the strain were determined.The results showed that the optimal condition for enzyme production fermentation was that the temperature was 35 C,pH value 7.0,initial PCBs concentration of 0.2 mg/L,250 rnl flask for 3 d.Enzymatic optimal active temperature was 35 C,the optimal active was pH 7.5.

关键词： polychlorinated biphenyls Bacillus subtilis WF1 enzyme

作者: WU Ke LIU Bin YU Zhimin JIN Jie Michael NELLES

作者单位: Hefei University Key Laboratory of Applied Enzymology & Engineering; Hefei Institute of Environmenta Department of Waste Management and Material Flow, Rostock University, 18059 Rostock, Germany

会议类型: 国际会议

会议名称: The 4th International Conference on Environmental Technology and Knowledge Transfer(第四届亚欧环境技术及知识转化国际研讨会(2012ICET))

会议地点: 合肥

会议语种:英文

页码: 533-535

在线出版日期: 2012-05-24（万方平台首次上网日期，不代表论文的发表时间）