Microbial Total DNA Extraction and Purification from Aerobic Compost
Total microbes DNA were extracted from vegetable waste compost and purified by spin-bind cartridges.The modified DNA extraction yielded more than 76±5.8 (μg crude DNA/g compost) and 61±4.5 (μg purified DNA/g compost),more than 98%humic acids were efficiently removed by the purification.The gel electrophoresis of purified DNA had a length of about 23kb,and the band intensity roughly reflected the changes of DNA genome content in compost processing.The amplification of 16S rDNA V3 region showed the purified DNA was sufficiently free of contaminants to allow PCR amplification.These results indicated that the modified method to extract and purify compost genomic DNA were efficient and could be used for molecular analysis of complex microbial communities.
Extraction Purification Aerobic compost Humic acids 16S rDNA
KAN Jinsong WU Ke Michael NELLES
Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China Department of Waste Management and Material Flow, Rostock University, 18059 Rostock, Germany
国际会议
合肥
英文
546-549
2012-05-24（万方平台首次上网日期，不代表论文的发表时间）