Optimization of Extraction Procedures for EPS Extraction of Desulfovibrio Desulfurican
The efficiencies of extracting extracellular polymeric substances (EPS) from Desulfovibrio desulfurican was compared by using ethylene diamine tetraacetic acid disodium salt (EDTA),cation exchange resin (CER),NaOH,H2SO4,heating and sonication.Results showed that heating was most effective in extracting EPS.The carbohydrate,protein and nucleic acid in the EPS solution were 4.83,12.62 and 4.80mg/g dry cells,respectively.Results indicated that the EPS content were greatly dependent on the extraction method.UV-visible spectrum of the EPS extraction suggested that few cells were destroyed and lysis did not occur.UV-visible spectrum analysis,an easy and rapid technique,can be used to monitor cell lysis during EPS extraction from D.desulfurican
extracellular polymeric substances (EPS) Desulfovibrio desulfurican extraction methods
WAN Zhengqiang YU Yanyun YU Zhimin
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009 Department of Biological & Environmental Engineering, Hefei University, Hefei 230022, China
国际会议
合肥
英文
642-646
2012-05-24（万方平台首次上网日期，不代表论文的发表时间）