Comparison of Seven Extraction Methods for Extracellular Polymeric Substances from Acidithiobacillus ferrooxidans
The efficiencies of extracting extracellular polymeric substances (EPS) from Acidithiobacillus ferrooxidans (A.ferrooxidans) using ethylene diamine tetraaeetic acid disodium salt (EDTA),cation exchange resin (CER),NaOH,H2SO4,heating method,sonication and centrifugation were compared.EPS constituents such as carbohydrate,protein,nucleic acid were analyzed.Results showed that EDTA method was most effective in extracting EPS from A.ferrooxidans.For each gram of dry cells,73.69mg of EPS was obtained.The quantification of EPS and the percentage of the three main components were strongly dependent upon the extraction methods.The UV-visible spectrum was also employed to be used as an efficient and convenient way to evaluate the extent of cell lysis for making a judgment on EPS extraction methods.
extracellular polymeric substances (EPS) Acidithiobacillus ferrooxidans extraction methods
YU Yanyun WAN Zhengqiang PENG Shuchuan JIN Jie
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009 Hefei Institute for Environmental Engineering, Hefei University, Hefei 230022, China
国际会议
合肥
英文
714-717
2012-05-24（万方平台首次上网日期，不代表论文的发表时间）