Porous Silicon Immunosensor for Label-free Detection of SpaA

摘要：

　　A novel immunosensor based on porous silicon (PSi) for antigen detection was reported in this paper.The antigen (SpaA) and the specificity of the antibodies are employed as the target and the probe in our laboratory,respectively.The immunosensor structure was prepared using bioconjungation.After the antigen-antibody reaction,the red shift of the reflection spectrum of the immunosensor increases in proportion to the concentration of SpaA.The sensitivity of this immunosensor is 41 nrn/μg·m1-1 and the detection limit is 2.44× 102pg·m1-1,they are better compared with our previous work by using this method.Controlled experiments were also presented with non-immune antibody and the results show that this immunosensor possesses high specificity.

关键词： porous silicon SpaA of Erysipelothrix rhusiopathiae immunosensor reflection spectrum label-free detection

作者: Tao Jiang Xiaoyi Lv Furu Zhong Jiaqing Mo Yixian Tu Zhenhong Jia Fuchun Zhang

作者单位: School of Physics, Xinjiang University, Urumqi 830046, China School of Electronic and Information Engineering, Xian Jiao tong university, Xian 710049, China Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and T College of Information Science and Engineer, Xinjiang University, Urumqi 830046, China

会议类型: 国际会议

会议名称: the 2010 International Conference on Frontiers of Manufacturing and Design Science(第一届制造与设计科学国际会议(ICFMD 2010))

会议地点: 重庆

会议语种:英文

页码: 2472-2476

在线出版日期: 2010-12-11（万方平台首次上网日期，不代表论文的发表时间）