A Primary Study on Methanotrophs Diversity in Zoige Wetland

摘要：

　　In this study,the authors investigated methanotrophs diversity in Zoige Wetland using pmoA gene as a molecular marker.The primer set of A189-mb661 was chosen to amplify the functional gene which was approximately 500bp.The cloning library was constructed according to the pmoA detected.The phylogenetic tree showed that all the clones detected were affiliated to type Ⅰ methanotrophs.Three novel clades (ZC-1,ZC-2,ZC-3 and ZC-4) were found to relate to none of the methanotrophs previously reported.All members within the type I were related to only two known genera,Proteobacterium and Methylobacter.According to the results shown in figure,it is strongly suggested that further studies are needed to be done to make our results more persuasive and sufficient.

关键词： Cloning library Diversity Methanotrophs pmoA Gene Zoige Wetland

作者: Juan-li YUN An-zhou MA Hong-xun ZHANG Guo-qiang ZHUANG

作者单位: Graduate school,Chinese Academic of Sciences,Beijing 100049,P.R.China Research center for Eco-Environment Sciences,Chinese Academic of Sciences,Beijing 100085,P.R.China

会议类型: 国际会议

会议名称: The 2nd China-Japan Graduate Student Forum -- Life, Environment and Energy (第二届中日研究生论坛--生命,环境与能源)

会议地点: 北京

会议语种:英文

页码: 88-92

在线出版日期: 2009-09-24（万方平台首次上网日期，不代表论文的发表时间）