Impact of DDT Stress on Laccase Production and Isozyme Patterns of Ectomycorrhizal Fungus Xerocomus chrysenteron
Four oxidative enzymes,which were considered as key enzymes in DDT biodegradation of fungi,were tested 15 days after cultivation in the solid culture.Three of these enzymes,laccase,polyphenol oxidase and peroxidase were observed in all the cultures.X.chrysenteron showed very strong laccase activity in both control and DDT treated cultures.The laccase activity increased along with raised DDT concentration,this result indicates laccase as a potential key enzyme during DDT biodegradation by X.chrysenteron.To study DDTs impact on laccase production,different amounts of DDT were added to the liquid culture media on the lOth day.After 20-day exposure,the laccase activity was significantly higher than control.Native-PAGE staining with ABTS illustrated the laccase isozyme patterns after 20-day exposure to DDT.It showed two laccases isoformed in both control and DDT treated cultures,while DDT-treated cultures also showed a third isoform.This result indicated the impact of DDT or its metabolites on laccase production and that laccase might be an important enzyme in DDTs biodegradation by X.chrysenteron.
DDT Ectomycorrhizal fungus Xerocomus chrysenteron Laccase Isozyme
Xiao LI Yi HUANG Xi ZHAO
College of Environmental Sciences and Engineering,Peking University,Beijing 100871,P.R.China
国际会议
The 2nd China-Japan Graduate Student Forum -- Life, Environment and Energy (第二届中日研究生论坛--生命,环境与能源)
北京
英文
217-221
2009-09-24（万方平台首次上网日期，不代表论文的发表时间）