Rapid Quantitative Detection of 6-Benzylaminopurine on Gold Nanoparticles Using Surface-Enhanced Raman Scattering
6-Benzylaminopurine (6-BA) as a kind of plant growth regulator has been widely used in bean sprout production.A portable Raman spectrometer,employing aggregated gold nanoparticles colloid substrate,was used to establish a new rapid and quantitative method for 6-BA residues analysis in this study.The results showed that the major Raman shifts of 6-BA extraction were at 738 cm-1,1001 cm-1,and 1318 cm-1 detected by surface-enhanced Raman spectroscopy,which corresponding to adenine ring breathing,Cphenyl-H bending in plane,and C-N stretching,respectively.The intensities of normalized characteristic band 1002 cm-1 (I1002 to I738) showed high correlation with 6-BA concentrations.The concentration linear range was 0.1-5 μg ?mL-1.The coefficient of determination (R2) was about 0.99,and RSDs calculated below 15%.The commercial samples collected from local market had been detected by SERS and HPLC methods meanwhile,SERS data showed that the 6-BA residue was 1.02 mg and 2.03 mg per kilogram of each sample,while they were 1.13 mg and 2.10 mg per kilogram of each sample,in the HPLC model,correspondingly.The prediction accuracy shows that SERS is a promising technique for rapid tracing analysis.And it will be suitable especially for on-site testing and quality control in field application.
surface-enhanced Raman scattering, 6-benzylaminopurine rapid detection
P.Zhang D.Zheng T.Lin R.Zhong
College of Life Science and Bioengineering, Beijing University of Technology, Beijing, P. R. China
国际会议
中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)
杭州
英文
1-5
2012-11-04（万方平台首次上网日期，不代表论文的发表时间）