A PDMS membrane biosensor for cellular detection
With new applications in the areas of healthcare – genetics,diagnostics and drug discovery; environmental and industrial monitoring; quality control as well as security and threat evaluation,the need for high throughput label-free multiplexed sensors for biological sensing has increased in the last decade.In this paper,a new surface stress-based polydimethylsiloxane (PDMS) membrane biosensor for cellular detection is designed and fabricated.Each biosensor consists of two membranes: active membrane and reference membrane.The geometric parameters of the PDMS membrane are optimized through the finite element (FE) analysis.One fiber optic interferometer biosensor test system is built to test the characteristics of the biosensor.The biosensor is further functionalized using 11 Mercapto 1 undecanoicacid (MUA: SH-(CH2)10-COOH) and tested in contact with cells — Escherichia coli (E.coli).The results of our experiments showed that cells can be detected based on the surface stress-based PDMS membrane biosensor.The new approach for cellular detection has good sensitivity and biocompatibility.
PDMS biosensor cellular detection E. coli
Shengbo Sang Wendong Zhang Pengwei Li Jie Hu Gang Li
MicroNano System research Center, Taiyuan University of Technology, Taiyuan, Shanxi, China;Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education,Taiyuan University of Technology, Taiyuan, Shanxi, China
国际会议
中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)
杭州
英文
1-6
2012-11-04（万方平台首次上网日期，不代表论文的发表时间）