Electric Impedance Spectroscopy for on-chip Analysis of Human Trabecular Meshwork Endothelium
It is reported the investigation of in vitro cultured human trabecular meshwork (TM) using electric impedance spectroscopy (EIS).TM is a critical determinant of intraocular pressure (IOP),the increase of which is the main cause of glaucoma.An array of interdigitated microelectrodes (IMEs) was fabricated for electric impedance sensing.Equivalent electric circuit model was proposed to study the cellular activities on the IMEs,showing the impedance of extracellular matrix (ECM) and the cell coverage ratio have distinct signatures on the spectrum.The utility of the EIS was demonstrated by treating TM cells during their proliferation with dexamethasone (DEX).The impedance measurement at 40 kHz showed that a higher DEX concentration can induce more severe inhibition of cell proliferation.The measurement was validated by AlamarBlue assay.The measurement provided a reasonable explanation of glucocorticoid induced glaucoma,where the glucocorticoid alters the metabolic and physiological functions of TM cells by inhibiting normal cell growth and proliferation.
Electric impedance spectroscopy interdigitated microelectrodes human trabecular meshwork primary open angle glaucoma
Wenwen Gu Yi Zhao
College of Engineering and Technology, Southwest University, Chongqing, 400715, P. R. China Department of Biomedical Engineering, The Ohio State University, Columbus, Ohio, 43210, USA
国际会议
中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)
杭州
英文
1-6
2012-11-04（万方平台首次上网日期，不代表论文的发表时间）