A Single Cells Patterning Approach for Human Promyelocytic Leukemia Cells
To get the detailed information of single cells and screen drugs effectively,especially to develop anti-cancer drugs in view of the cellular heterogeneity of tumors,biochips on single-cell level are necessary.Based on the fabrication techniques of MEMS,substrates with areas of hydrophilicity and hydrophobicity were fabricated to pattern single cells.The optimal process parameters for substrates fabrication are obtained through a series of experiments.MEMS substrates with clear binary surface patterns of hydrophilicity and hydrophobicity are achieved and a general streptavidin template with high bio-compatibility was prepared.In the experiment,biotin-BSA was employed as adhesive proteins Followed by streptavidin and biotinylated antibodies.An array of single HL-60 (Human Promyelocytic Leukemia Cells,HL-60) cells was obtained based on above protein adsorption substrate.The factors affecting the result of the single-cells patterning were investigated and optimized,by which a relatively high patterning rate is achieved.To keep cells characteristics and also for the future parallel patterning of different cells,specific antibodies are investigated for the cell HL-60,including CD45 and CD15.The result of antibodies immobilization and patterning are presented,which can well match the designed template.
cellular heterogeneity single-cell arrays MEMS substrates single cells patterning
Dahai Ren Mingyang Cui Jun Wang Yiqiu Xia Zheng You
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments and Mechanics, Tsinghua University, Beijing, 100084, China
国际会议
中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)
杭州
英文
1-7
2012-11-04（万方平台首次上网日期，不代表论文的发表时间）