Microfluidics design for single cell detection

摘要：

　　Here we demonstrate a microfluidic-based analysis system based on single cell capture array,which can physically trap individual cell using μm-sized structures.A stable and in vivo-like microenvironment was built with the novel structure at the single-cell detection level.The microfluidic-based design can decouple single cells from fluid flow with the help of micropillars.The size and geometry of the cell jails are designed in order to discriminate between mother and daughter cells.It provides an experimental platform to efficiently monitor individual cell state for a long period of time.Furthermore,the parallel microfluidic array can ensure accuracy.In addition,finite element method (FEM) was employed to predict fluid transport properties for the most optimal fluid microenvironment.

关键词： microfluidic finite element analysis method cell detection cell capture

作者: Shaobo Du Wendong Zhang Shengbo Sang Jie Hu Pengwei Li Gang Li Hui Feng

作者单位: MicroNano System research Center, Taiyuan University of Technology, Taiyuan, Shanxi, China;Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education,Taiyuan University of Technology, Taiyuan, Shanxi, China

会议类型: 国际会议

会议名称: 中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)

会议地点: 杭州

会议语种:英文

页码: 1-5

在线出版日期: 2012-11-04（万方平台首次上网日期，不代表论文的发表时间）