Separation and Detection of Urinary Proteins by Two-dimensional Non-gel Sieving Electrophoresis on the Microfluidic Chip

摘要：

　　A novel separating and detecting method of urinary proteins by two-dimension non-gel sieving electrophoresis (NGSE) on the microfluidic chip was proposed in this paper.Human serum albumin (HSA) and human transferrin (TRF) labeled by FITC were mixed and taken as the synthesized urinary protein samples.Factors affecting on separation,such as the rate of voltage applied in two-dimension,the addition in the buffer,the concentration and pH of buffer and so on,were optimized.i.e.10% (w/v) dextran,0.5% ethanediamine,0.2mol/L Tris-boric acid,pH 9.3,the ratio of the applied electrical field in two dimensions 1:4.8 (25V:120V),respectively.FITC-HSA and FITC-TRF were efficiently separated in 2 min,and the resolution of two proteins achieved 2.2 on the designed microfluidic chip.Then,the urine samples of nephropathy patient were analyzed by the proposed two-dimensional NGSE on the microfluidic chip.The results matched well with those detected by common clinical methods.

关键词： non-gel sieving electrophoresis microfluidic chip urinary proteins

作者: Yanan Ni Jun Gan Yi Xu Zhitao Chen Changfeng Yang

作者单位: Chemistry and Chemical Engineering College, Chongqing University, Chongqing , China;Defense Key Disc Chemistry and Chemical Engineering College, Chongqing University, Chongqing , China

会议类型: 国际会议

会议名称: 中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)

会议地点: 杭州

会议语种:英文

页码: 1-6

在线出版日期: 2012-11-04（万方平台首次上网日期，不代表论文的发表时间）