StudStudy of Microfluidic Cell Chip for the toxicity test of Artemisinin on Hep-G2 cells
A novel microfluidic chip composed of an 6 × 6 array of cell culture microchambers was designed and fabricated in this paper.The microfluidic cell chip analytical system combined with the designed microchip,measuring device and environmental control unit was established for cell culture and parallel drug cytotoxicity assay.Human hepatoma cell Hep-G2 and artemisinin were taken as the objective samples in the experiments.HepG2 cells were successfully cultured in the microchip for over 72 hours.And viability percentage of the HepG2 cells was higher than 90% with staining of trypan-blue.The Hep-G2 cells were exposed to the artemisinin solutions at different concentrations ( 0、10、25、50、100、150μmol? L-1) for 12h,24h and 48h,respectively.Viability percentage of the HepG2 cells in the microchambers was measured by observing cellular morphology and fluorescence intensity.The results showed that artemisinin could inhibit the activity of HepG2 cells,which depended on the dose of artemisinin and action time.The microfluidic chip and system could not only solve the shortcomings of conventional in vitro cell culture,such as labor-intensive,reagent-consuming and time-consuming,but also improve great potential for the study of cell biology in the directions of miniaturization,automation,high efficiency and high sensitivity.
microfluidic cell chip Human hepatoma cell Hep-G2 Artemisinin cell toxicity test
Yao Wang Yi Xu Zhen Zhou Whenjuan Li Chunyan Dong He Liu Lianguo Zhao
Chemistry and Chemical Engineering College, Chongqing University,Chongqing , China;Defense Key Disci Chemistry and Chemical Engineering College, Chongqing University,Chongqing , China;Defense Key Disci Chemistry and Chemical Engineering College, Chongqing University,Chongqing , China
国际会议
中国微米纳米技术学会第14届学术年会、第3届国际年会暨第6届微米纳米技术“创新与产业化国际研讨与展览会(CSMNT2012 & ICMAN2012)
杭州
英文
1-6
2012-11-04（万方平台首次上网日期，不代表论文的发表时间）