Study of Collagen/Silk Nanofiber Scaffold for Hepatocyte Culture

摘要：

　　Collagen/silk nanofiber scaffold was prepared via electrospinning,and human hepatoma cell HepG2 had been cultured with it.The cell’s morphological change,viability and other functions were then investigated.Taking 1,1,1,3,3,3- hexafluoro-2-propanol(HFIP) as solvent,using electrospinning to prepare the collagen/silk nanofiber scaffold,and the mass ratio of collagen/silk fibroin as 100:0,70:30,50:50,30:70,0:100.The scaffold’photo of scanning electron microscopy (SEM) showed the average diameter of fibers were range from 550nm to 1100nm.And fiber diameter was positively correlated with SF content.Cell culture results shows HepG2 cell grew well on the surface of materials and closely with the scaffold material.The conventional cultured cells gradually die after 5 days and loss the cell function,the collagen / silk nanofiber scaffold group cells can maintain a steady state in 4-9 days,and the urea synthesis and albumin secretion have significant differences with conventional culture group.The nanofiber scaffold with silk fibroin (SF) content of 50% has better cell state and cell function than other groups.Compared with the conventional culture group,HepG2 cell grows well on the collagen/silk nanofiber scaffold and maintain the functionexpression in longer time.The collagen/silk nanofiber scaffold can be expected use in bioartificial liver to improve liver bioreactor ‘s cell activity and maintain the cell function expression in longer time.

关键词： bioartificial liver electrospinning,collagen,silk fibroin

作者: HU Wei LIU Xin GU Hanqing

作者单位: Department of Biomedical Engineering, Tianjin Medical University, Tianjin, China Institute of Urological Surgery,Tianjin,China

会议类型: 国际会议

会议名称: World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))

会议地点: 北京

会议语种:英文

页码: 95-98

在线出版日期: 2012-05-26（万方平台首次上网日期，不代表论文的发表时间）