Modification of PLGA Scaffolds for Vascular Tissue Engineering

摘要：

　　We report this modification of poly(lactic-coglycolic acid) (PLGA) scaffolds,both to impart hemocompatibility to prevent platelet adhesion and aggregation before the endothelial confluence is fully achieved,and to support EC growth to accelerate endothelialization.The modification was achieved by covalent immobilization of sulfated silk fibroin on PLGA scaffolds using gamma irradiation.The systematic in vitro hemocompatibility evaluation revealed that sulfated silk fibroin covalently immobilized PLGA (S-PLGA) scaffolds reduced platelet adhesion and activation,prolonged whole blood clotting time,activated partial thromboplastin time (APTT),thrombin time (TT),and prothrombin time (PT).Vascular ECs were seeded on the scaffolds and cultured for 2 weeks.The ECs were seen to attach and proliferate well on S-PLGA scaffolds,forming cell aggregates that gradually increased in size and fused with adjacent cell aggregates to form a monolayer covering the scaffold surface.Moreover,it was demonstrated through the gene transcript levels and the protein expressions of EC-specific markers that the cell functions of ECs on S-PLGA scaffolds were better preserved than those on PLGA scaffolds.

关键词： Hemocompatibility Sulfated silk fibroin Covalent immobilization Vascular grafts

作者: Yanxue Bi Xili Ding Gang Zhou Haifeng Liu Yubo Fan

作者单位: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering,Beihang University, Beijing 100191, Peoples Republic of China

会议类型: 国际会议

会议名称: World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))

会议地点: 北京

会议语种:英文

页码: 110-112

在线出版日期: 2012-05-26（万方平台首次上网日期，不代表论文的发表时间）