Microfluidic Carbonylated Protein Capture

摘要：

　　We report a proof of principle study for the use of oxalyldihydrazide as a crosslinker for enrichment of carbonylated proteins within a microfluidic chip.Surface modification steps are characterized and analyzed using analytical techniques.We use in vitro oxidized bovine serum albumin as a model protein for testing the selectivity and specificity of the methodology for carbonylated proteins.We demonstrate high specificity for our target protein,obtaining 100 % capture of our target molecule during the first three minutes of continuous injection.After 30 minutes of continuous loading,the device captures half of the targeted molecules.Elution of the proteins is confirmed qualitatively.Under the flow conditions used in this experiment,nearly 300 ng of proteins can be enriched prior to chip saturation.This report suggests the validity of using immobilized oxalyldihydrazide on a microchip as an enrichment methodology for low abundance proteins in a sample.

关键词： BioMEMS carbonylation microfluidic enrichment oxalyldihydrazide protein capture

作者: Bryant C. Hollins Steven A. Soper June Feng

作者单位: Department of Biomedical Engineering, Louisiana Tech University, Ruston, LA, USA Department of Chemistry, Louisiana State University, Baton Rouge, LA, USA

会议类型: 国际会议

会议名称: World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))

会议地点: 北京

会议语种:英文

页码: 270-273

在线出版日期: 2012-05-26（万方平台首次上网日期，不代表论文的发表时间）