Detection of bacteria based on quantum dots by using a laboratory-made system

摘要：

　　This study investigated a method that simultaneously detects three bacteria,Salmonella typhimurium,Escherichia coli,and Staphylococcus aureus via an approach that use antibody-conjugated quantum dots (QDs) as fluorescence markers,by using a laboratory-made system.QDs with different emission wavelenghs were immobilized with antibody.Antibody conjugated QDs capture the bacteria selectively and specifically so that “sandwich complex were formed.The suspension of the labeled bacteria was trickled onto a microporous membrane.A 450nm semiconductor laser was used as a part of the laboratory-made system to excite the QDs.Three PMT detectors were utilized to detect the fluorescence intensity.This method,of which the detection procedures are completed within 1 h,can be applied to the cost-effective and rapid detecting of bacterial contamination.

关键词： Quantum dot fluorescent detection

作者: Zhi Cheng Taihu Wu Feng Chen Yaohua Du Biao Gu Chao Li Zijian Yang

作者单位: Institute of Medical Equipment, Tianjin, China

会议类型: 国际会议

会议名称: World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))

会议地点: 北京

会议语种:英文

页码: 278-281

在线出版日期: 2012-05-26（万方平台首次上网日期，不代表论文的发表时间）