Cell Trapping and Detection by Dielectrophoresis with SU-8 Grooves
In this paper,a novel dielectrophoretic microfluidic device is proposed for cell trapping and detection.The dielectrophoresis device consists of inter-digital electrodes and SU-8 grooves.The SU-8 structure with grooves was fabricated on the top of the electrodes with the strongest electric field formed at the edges of the exposed electrodes.Cells can be trapped in the SU-8 grooves by positive dielectrophoretic force and released back to the fluid flow by negative dielectrophoretic force.The height of the grooves is designed such that the cells remained trapped and cannot be flushed away by the fluid flow when the electric field is turned off.This allows further cell analysis to be conducted in a saline buffer with the electric field turned off,avoiding the electrodes from getting burned in a high conductivity medium.The trapping and detection process is demonstrated using human colon cancer cells (HT-29 cells) and cryptosporidium parvum.Viability test in a phosphate buffer was conducted after HT-29 cells are trapped by the grooves.The capture of cryptosporidium parvum oocyst cells in water followed by cell specific detection by anti-cryptosporidium antibody is also reported.
Dielectrophoresis groove trap cancer cell cryptosporidium parvum
Liqun Wu Lin-Yue Lanry Yung Kian-Meng Lim
Department of Mechanical Engineering, National University of Singapore, Singapore Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore Department of Mechanical Engineering, National University of Singapore, Singapore;Singapore-MIT Alli
国际会议
World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))
北京
英文
305-308
2012-05-26（万方平台首次上网日期，不代表论文的发表时间）