The Observation of the PG-depletion in Progressive Trypsin Digested Articular Cartilage Based on Conventional A-mode Ultrasound
This study tried to monitor proteoglycans (PG)-depletion in progressive trypsin digested porcine articular cartilage (AC) samples with a conventional ultrasound system.10 cartilage-bone samples were prepared from normal porcine femoral condyle cartilages and were digested with 0.25% trypsin solution for two hours.The dynamic process of the PG digestion was explored using an A-mode ultrasound experiment system with lOMHz center frequency.The speed of PG-depletion and the degree of PG-depletion were estimated from ultrasound echo signal.The results showed that lOMHz ultrasound is valuable in tracking the degree of PG-digestion.The transient speed of PG-digestion decreased with digesting time and tissue depth.The digestion speed is faster in the superficial layer of the cartilage samples (0.93±0.3M/min) than that in deeper layers (0.26±0.12 M/min at 50% of the full thickness).The histological image also confirmed the validity of the ultrasound observation..This study may provide some useful information in identifying c artilage matrix integrity and osteoarthritis (OA) disease progression.
Articular cartilage Conventional Ultrasound Trypsin degeneration PG-depletion Osteoarthritis
WANG Li NIU Haijun YAN Yan SUN Feng LI Deyu FAN Yubo
School of Biological Science and Medical Engineering, Beihang University, Beijing, China
国际会议
World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))
北京
英文
1101-1104
2012-05-26（万方平台首次上网日期，不代表论文的发表时间）