Fractal analysis of rat bone marrow mesenchymal stem cells during differentiation into neural cells in vitro
Fractal dimension (FD) is a good option to quantitatively describe morphological complexity of cells.During the differentiation of rat bone marrow mesenchymal stem cells (rBMSC) into neural cells,morphological complexity of rBMSC increases,so FD can be a useful feature to characterize cell shape complexity at any given point in time during the differentiation process.In this study rat BMSCs were isolated and differentiated to neural cells.During the differentiation process,microscopic images were taken at certain intervals.After applying proper image processing algorithms implemented in Matlab software,FDs of the cell images were calculated by means of boxcounting method.Results demonstrated that FD of the recorded images had an ascending trend after one hour of neural induction,and simultaneously cells phenotype started changing from spindle shape to neural-like phenotype with multiple branches.Therefore,a direct correlation between cell shape complexity and FD of the images was observed,and it proves that FD serves as a practical method for quantitatively describing complexity as a morphological feature of the cells during differentiation.
Fractal dimension differentiation neural cell cell morphology bone marrow mesenchymal Stem cells
M. Kazemi Moghadam M. Janmaleki M.H. Fouani S. Abbasi M. Marandi
Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences,
国际会议
World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))
北京
英文
1965-1968
2012-05-26（万方平台首次上网日期，不代表论文的发表时间）