Evaluation of the performance of a novel hydrostatic bioreactor
pH of cell culture medium and dissolved oxygen are important criteria for cell proliferation and differentiation and are also crucial for the performance of cell culture systems.Therefore,this study aims to investigate the performance of a novel hydrostatic bioreactor while measuring the changes of pH and dissolved oxygen in bicarbonatebuffered and HEPES-buffered cell culture media.pH changes of media samples,which were subjected to hydrostatic pressure with varying pressure amplitudes,frequencies and exposure times,were determined via a colorimetric approach.The concentration of dissolved oxygen was measured using a micro optical oxygen sensor.An increase in pressure,frequency and time resulted in an increase on pH.However,after an exposure time of 30 minutes no further increase in pH was observed and the pH of the media samples were adjusted to the cell culture environment of the incubator.Similar trends were observed for bicarbonatebuffered and HEPES-buffered media.Initial results suggest further that pressure had a stronger influence on pH than frequency.
hydrostatic pressure bioreactor dissolved oxygen measurement pH measurement
Y. Reinwald A. J. El Haj
Institute of Science and Technology in Medicine, University of Keele, Thornburrow Drive, Hartshill, Stoke-on-Trent ST4 7QB, UK
国际会议
World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))
北京
英文
1969-1972
2012-05-26（万方平台首次上网日期，不代表论文的发表时间）