Unidirectional Pulsatile Flow Improves Fibronectin and Laminin Reorganization and Cell Adherence of Vascular Endothelial Cell
Cell adherence is a key factor in endothelialization of prosthetic vascular grafts and artificial valves.Flow conditions are proved to affect fibronectin (FN) and laminin (LN) organization of vascular endothelial cells (ECs),thereby changing ECs adherence.The blood flow in aortic is pulsatile,and the flow pulsatility is critical in ECs functions.However,effects of pulsatile flow on FN and LN organization and cell adherence of ECs are still unclear.In this study,rat aortic endothelial cells (RAECs) were exposed to steady laminar flow of 12 dyne/cm2 and unidirectional sinusoidal flow with equivalent time-average shear stress of 12 dyne/cm2 and a frequency of 1.25 Hz for 24hours,and then FN and LN reorganization and cell retention were investigated.Compared to steady laminar flow,FN fibrils under unidirectional pulsatile flow appeared to be thicker and formed a denser network.LN under unidirectional pulsatile flow formed in fibrillar pattern,no matter in the intercellular space or beneath the cells,and the LN fibrils tended to orient more regularly in the flow direction.After exposure to 40 dyne/cm2 continuous laminar shear stress for 2h,4h and 6h,the cell retention ratio under unidirectional pulsatile flow was much higher than steady laminar flow group.These results indicated that unidirectional pulsatile flow improves RAECs adherence by reorganization of FN and LN.
fibronectin laminin cell adherence unidirectional pulsatile flow reorganization
Xianghui Gong Lihua Xie Lin Li Fan Zhang Changhui Ren Yubo Fan
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University
国际会议
World Congress on Medical Physics and Biomedical Engineering (2012年医学物理及生物医学工程国际会议(IFMBE))
北京
英文
1991-1994
2012-05-26（万方平台首次上网日期，不代表论文的发表时间）