HPLC Method Research of Picoplatin-a New Platinum Anti-cancer Drug and Its Impurities
Purpose: To establish a HPLC testing method of the content of bulk picoplatin and its impurities. Method: the separation was perform on a C18 column(4.6 mmx250 mm, 5 urn) with potassium dihydrogen phosphate-aceton-itrile as the mobile phase at a flow rate of 1.0 mL/min. The detecting wavelength was set at 210 nm, and the column temperature was set at 30℃. Result: in the method validation, the linear relationship modulus of picoplatin is 0.9999, the systemic precision is 0.44％, the method precision is 0.74％, the average recovery rate is 99.62％, the LOD and LOQ of picoplatin is 0.2 ng and 1.0 ng. The average resolution of picoplatin and its impurities is more than 2. Conclusion: The established method is good specificity, high sensitivity, and good repeatability which could provide scientific evidence for the quality control of picoplatin and its impurities.
platinum-based anti-cancer drug picoplatin HPLC
LUAN Chunfang CONG Yanwei ZHANG Qi QIU Xueweng, BEI Yuxiang PU Shaoping
Kunming Guiyan Pharmaceutical Co. Ltd., Kunming 650106, China
国际会议
5th International Conference Platinium Metals(第五届国际贵金属会议)
昆明
英文
253-257
2012-10-15（万方平台首次上网日期，不代表论文的发表时间）