Factors Affecting Phase I Stereoselective Biotransformation of Chiral Persistent Organic Pollutants (POPs) by Cytochrome P-450 Isozymes

摘要：

Factors affecting cytochrome P-450 (CYP) isozymemediated phase I stereoselective biotransformation of chiral persistent organic pollutants (POPs) were investigated using chiral polychlorinated biphenyls (PCBs) (PCBs 45, 95 and 132) as probe compounds. Rat CYP 2B1 preferentially biotransformed the secondeluting atropisomers of PCBs 45 and 95 at low substrate concentrations (≤ 15 μmol/L). Competition by different congeners decreased the biotransformation rates of each congener stereoselectively, affecting atropisomeric composition, with increasing competition for congeners with greater chlorination. No atropisomeric enrichment was observed for PCB 132 upon incubation of the racemate. However, under the same conditions, significant differences in biotransformation kinetics were observed in individual atropisomer incubations, indicating that ( + )-PCB 132 and (-)-PCB 132 competed to be biotransformed.

关键词： chiral persistent organic pollutants stereoselective biotransformation cytochrome P-450 isozyme competition and inhibition nanoparticles

作者: Zhe Lu Charles S.Wong

作者单位: Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada Richardson College fo

会议类型: 国际会议

会议名称: Proceedings of 4th Internatioanl Symposium on Pesticides and Environmental Safety & 5th Pan Pacific Conference on Pesticide Science & 8th International Workshop on Crop Protection Chemistry and Regulatory Harmonization (四届农药与环境安全会)

会议地点: 北京

会议语种:英文

页码: 98

在线出版日期: 2012-09-15（万方平台首次上网日期，不代表论文的发表时间）