RNA Interference-mediated Knockdown of Several Components of Cytochrome P450 Enzyme Systems, from the Cotton Bollworm,Helicoverpa armigera , Enhances Larval Susceptibility to beta-Cypermethrin

摘要：

The cotton bollworm, Helicoverpa armigera (Hiibner), is an important agricultural pest, making severe yield losses in many crops. Its development of resistance to various agrochemicals has become a serious problem. Previous studies reported that enhanced metabolic activity of cytochrome P450 enzyme systems (P450s) was proved to be a major mechanism of insecticide resistance. Recently, the authors found that knockdown of CYP6B7, cytochrome b5 (Cyt-b5) and NADPH-dependent cytochrome P450 reductase (CPR) in the fenvalerate-resistant HDFR strain of H. armigera by RNA interference (RNAi) via injection of doublestranded RNA (dsRNA), increases larval susceptibility to fenvalerate. In the present study, the functional interactions of CYP6B7, CPR and Cyt-b5 were further assessed using the same strategies and monitoring for resistance levels of beta-cypermethrin.

关键词： Helicoverpa armigera beta-cypermethrin cytochrome P450 enzyme systems insecticide resistance RNA interference

作者: Tao Tang Chunqing Zhao Xiaoyun Feng Xueyuan Liu Lihong Qiu

作者单位: Key Laboratory of Pesticide Chemistry & Application Technology, Ministry of Agriculture, College of Key Laboratory of Pesticide Chemistry & Application Technology, Ministry of Agriculture, College of Institute of Plant Protection, Hunan Academy of Agricultural Sciences,Changsha, China

会议类型: 国际会议

会议名称: Proceedings of 4th Internatioanl Symposium on Pesticides and Environmental Safety & 5th Pan Pacific Conference on Pesticide Science & 8th International Workshop on Crop Protection Chemistry and Regulatory Harmonization (四届农药与环境安全会)

会议地点: 北京

会议语种:英文

页码: 416-417

在线出版日期: 2012-09-15（万方平台首次上网日期，不代表论文的发表时间）