Monoclonal Antibody Binding to (Fluoro) Quinolones in Competitive Immunoassay: Quantitive Correlation Antibody Affinity with Molecular Parameters
Antibodies used in competitive immunoassay tor the detection ot small molecule usually recognize one more molecules that share common epitopes with different affinities. For immunochemist, there is a challenging problem to well understand the cause of the antibody s multi-specificity from only looking two-dimensional structural formulas of the compounds studied (Sanvicens et al. , 2003). A method that can provide useful parameters about the topological and electrostatic properties of molecule can be sufficient in elucidating the diversity of antibody binding (Beier and Stanker 2001). In the study, we determinates the affinities of one monoclonal antibody (C4A9H1) to 14 (fluoro) quinolones by competitive ELISA and calculated molecular parameters of these (fluoro)quinolones by quantum chemical B3LYP/6-31G*.
monoclonal antibody (fluoro)quinolones immunoassay antibody affinity molecular parameters
Zhanhui Wang
Department of Veterinary Pharmacology and Toxicology, college of Veterinary Medicine, China Agricultural University, Beijing 100193, China
国际会议
北京
英文
449-450
2012-09-15（万方平台首次上网日期，不代表论文的发表时间）