Factors Affecting Callus Induction on Anther Culture of Proso Millet (Panicum miliaceum L.)
Proso millet is a promising species for rich nutrition, drought and barren-tolerance. Influences factors on anther-derived callus induction efficiency were investigated when microspores of tested anthers at the late-uninucleate to early-binucleate stage were used as explants. The results indicated the callus induction was strongly affected by genotype. Several highly responsive cultivars of proso millet with desired traits had been identified and they could be directly used in crop improvement by breeding. Cold pre-treatment for three weeks of spikes then 32.5℃ shortly heat post-incubation culture could significant improve callus yield. Moreover, W14 basal medium and combination 60 g/L sucrose with 60 g/L maltose as carbon source were optimal for anther culture of proso millet in this study. To our best knowledge, this is the first report on successful induction of pollen-derived callus in proso millet and it will accelerate the breeding programs.
Proso millet Temperature Medium Genotype Carbohydrate
Wu Limin Yang Tianyu Dong Guojun He Jihong Dong Kongjun Wang Huizhong Yu Yanchun
College of Life Science,Hangzhou Normal University,Hangzhou 310036 China Crop Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070 China State Key Laboratory of Rice Biology,China National Rice Research Institute Hangzhou,Zhejiang 310006 ollege of Life Science,Hang zhou Normal University,Hangzhou 310036 China
国际会议
The 1st International Symposium on Broomcorn Millet(第一届国际糜子学术研讨会)
西安
英文
125-135
2012-08-25（万方平台首次上网日期，不代表论文的发表时间）