Isolation and Analysis of Genes Induced by Rehydration after Serious Drought in Broomcorn Millet (Panicum miliaceum L.) by Using SSH
In order to investigate the molecular mechanism of rehydration after serious drought in broomcorn millet (Panicum miliaceum L.), a forward subtracted cDNA library was constructed between normal watered leaves and rehydrated leaves after serious drought conditions, using the suppressive subtraction hybridization (SSH) technique. A total of 60 positive clones were randomly picked out from the subtracted library and sequenced, and redundancy sequences were removed after sequence alignment. Based on the results of sequence homologous comparison and function querying, 32 expressed sequence tags (EST) were highly homologous with known EST. Most of those sequences were related to either abiotic or biotic stresses in plants. Of those sequences, 11 ESTs were homologous with ESTs in rat (Rattus norvegicus) liver after partial hepatectomy. The Blast result of proteins revealed that 28 ESTs were similar to known proteins. The functions of these proteins mainly involve signal transduction, transcription and protein processing. This experiment demonstrated that a range of specific genes was induced and expressed in broomcorn millet during the rehydration stage after serious drought.
Broomcorn millet Drought stress Rehydration Gene expression Suppressive subtraction hybridization (SSH)
Lin Fanyun Hu Yingang Song Gouqi Zhang Hong Liu Tianming He Beiru
College of Agronomy,Northwest A & F University,Yangling 712100,China College of Agronomy, Northwest A & F University,Yangling 712100,China Yangling Branch of China Natio College of Agronomy, Northwest A & F University,Yangling 712100,China Yangling Branch of China Natio
国际会议
The 1st International Symposium on Broomcorn Millet(第一届国际糜子学术研讨会)
西安
英文
136-145
2012-08-25（万方平台首次上网日期，不代表论文的发表时间）