Analysis of Protein Component of Proso Millet Grain
This study analyzes the grain protein components of proso millet to provide a theoretical basis for proso millet germplasm research and breed improvement. 13 proso millet lines were selected in the trials to extract albumin, globulin, gliadin and glutenin. Coomassie brilliant blue method was used to analyze the content of them, and SDS-PAGE was used to analyze of subunit composition of them. The results showed that the average content of albumin in the tested varieties grain were 13.67 mg/g, globulin 8.12 mg/g, gliadin 2.70 mg/g and glutenin 8.12 mg/g. In the four components, the difference of gliadin content was significantly between varieties. The SDS-PAGE showed that the bands of protein components could be seen clearly after dyeing. Between 14.4 ku and 94.0 ku, the number of bands of albumin ranged from 10 to 12, whereas Globulin was 9-11, glutenin was 10 or 11. The result showed that the surplus protein was very rich.
Proso millet Protein opponent SDS-PAGE Diversity
Zhang Zhifen Gao Xiaoli Feng Baili Chai Yan Dang Genyou Xie Huimin
Inner Mongolia Academy of Agricultural and Husbandry Sciences,Inner Mongolia,010031 College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China College of Agronomy,Northwest A&F University, Yangling,Shaanxi 712100,China College of Agronomy,Northwest A&F University,Yan gling,Shaanxi 712100,China
国际会议
The 1st International Symposium on Broomcorn Millet(第一届国际糜子学术研讨会)
西安
英文
330-335
2012-08-25（万方平台首次上网日期，不代表论文的发表时间）