Inhibition of Taq DNA polymerase and DNA exonuclease Exo III by an aqueous nanoparticle suspension of a bis-methanophosphonate fullerene

摘要：

An aqueous nanoparticle suspension of a bis-methanophosphonate fullerene (n-BMPF) was tested to clarify its effects on polymerase chain reaction (PCR) with the catalyst of Taq DNA polymerase and DNA exonuclease Exo III and the template of super-coiled plasmid pEGFP-N1. It was found that the product amounts from PCR decreased significantly with addition of the n-BMPF. The inhibition by the n-BMPF was dose-dependent and IC50 values for reactions of PCR were 2.7 μmol/L. Increase of Taq DNA polymerase amounts in PCR system antagonized the activities of the n-BMPF. However, addition of two scavengers of reactive oxygen species (ROS), mannitol and azide at the concentrations of 2~10 mmol/L did not antagonize the activities of the n-BMPF against PCR. These data implied that this inhibition probably did not correlate to ROS. Meanwhile, the inhibition for the DNA exonuclease Exo III by the n-BMPF was evident and dose-dependent.

关键词： bis-methanophosphonate fullerene aqueous nanoparticle suspension DNA exonuclease reactive oxygen species

作者: KANG Feng SONG Gaoguang

作者单位: School of Life Science, Beijing Institute of Technology, Beijing 100081, China Beijing Laboratory An School of Life Science, Beijing Institute of Technology, Beijing 100081, China

会议类型: 国际会议

会议名称: 11th IUMRS International Conference in Asia(第十一届国际材联亚洲材料大会 IUMRS-ICA 2010)

会议地点: 青岛

会议语种:英文

页码: 345-351

在线出版日期: 2010-09-25（万方平台首次上网日期，不代表论文的发表时间）