Effect of Environmental Factors of Mannanase on Konjac Glucomannan Molecular Dimension

摘要：

The environmental factors of mannanase konjac glucomannan (KGM) have been investigated in aqueous solution. Molecular weight (Mw) and molecular weight distribution (WMD) of the degraded KGM were measured by GPC. During the degradation process, the molecular weight of the enzymatic products decreases with reaction time, degradation reaction combining two reactions processed with different orders, while WMD broadens at first and then becomes narrow. The reduction of molecular dimension of KGM was lowered with temperature increase, but WMD increases. WMD was reduced with concentration of enzyme increase, but the reduction of molecular dimension of KGM was insignificant in the range of 2.0~6.5 u.mL-1 of mannanase concentration. The results show that molecular dimension of KGM could be controlled by soft changes of reaction environment, dimension of KGM molecule could be decreased more than 100 times so as to obtain oligosaccharides, which offers the reference to reliable theoretical foundations and practical ground in exploiting KGM potential function and activity.

关键词： Kongjac glucomannan Mannanase Molecular dimension Molecular weight distribution Temperature

作者: LONG XiaoYan LUO XueGang BAI Ju ZHU JiaFeng

作者单位: School of Life Science and Engineering, Southwest University of Science and Technology,Mianyang 6210 School of Life Science and Engineering, Southwest University of Science and Technology,Mianyang 6210 School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621

会议类型: 国际会议

会议名称: 11th IUMRS International Conference in Asia(第十一届国际材联亚洲材料大会 IUMRS-ICA 2010)

会议地点: 青岛

会议语种:英文

页码: 308-314

在线出版日期: 2010-09-25（万方平台首次上网日期，不代表论文的发表时间）