Photosynthetic Measurements with the Idea Spec: an Integrated Diode Emitter Array Spectrophotometer/Fluorometer
In vivo spectrophotometry, a non-invasive, nondestructive technique that relies on the leafs endogenous chromophores, has become an essential tool for understanding the photosynthetic response of plants to environmental stresses. Based on the pulsed-light spectrophotometer approach, and capitalizing on recent advances in optics and light emitting diode (LED) technology, we have developed an in vivo spectrophotometer capable of measuring absorbance changes of less than 3 × 10-5 absorption units and microsecond time resolution. The instrument can also simultaneously measure chlorophyll (or other) fluorescence signals with background or saturating actinic light, e.g. PAM fluorometry or induction curves, to give measurements of antenna and Photosystem Ⅱ efficiencies. We use a solid-state light source containing multiple LEDs for both measuring and actinic stimulation and direct the light to the leaf through non-focusing optics, allowing nearsimultaneous multi-wavelength measurements useful for signal deconvolution.
Spectrophotometry Fluorometry Spectroscopy Electron transfer chain Light reactions
Christopher C Hall Jeffrey Cruz Magnus Wood Robert Zegarac Dustin DeMars Joel Carpenter Atsuko Kanazawa David Kramer
Washington State University, Institute of Biological Chemistry, Pullman, WA Michigan State University, Plant Research Lab, East Lansing, MI
国际会议
15th International Conference on Photosynthesis(第15届国际光合作用大会)
北京
英文
184-188
2010-08-22（万方平台首次上网日期，不代表论文的发表时间）