Direct Detection of Oxygen Ligands to the Mn4Ca Complex in Photosystem Ⅱ by X-ray Emission Spectroscopy

摘要：

The Mn4CaOx cluster catalyzes the light driven oxidation of water in Photosystem Ⅱ. To understand the mechanistic details of this intriguing machine it is necessary to unravel not only the geometric but also the electronic structure of this complex. X-ray emission spectroscopy can be one important tool in this endeavour due to its unique sensitivity towards light atoms ligated to a metal center. In addition Xray emission spectroscopy provides information of the charge density distribution over the metal centers in the complex and is sensitive to protonation changes of bridging oxygens, both important questions in the light of different proposed mechanisms for water oxidation in PSⅡ. Here we show that X-ray emission spectra of PSⅡ can be collected with sufficient S/N from PSⅡ samples and can yield insights into the mechanism of water oxidation.

关键词： Photosystem Ⅱ Water oxidation X-ray spectroscopy X-ray emission spectroscopy

作者: Jan Kern Henning Schroeder Megan Shelby Yulia Pushkar Benedikt Lasalle Pieter Glatzel Vittal K Yachandra Uwe Bergmann Junko Yano

作者单位: Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA Lawrence Berkeley National Laboratory,Berkeley, CA 94720, USA European Synchrotron Radiation Facility, Grenoble, France SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA

会议类型: 国际会议

会议名称: 15th International Conference on Photosynthesis(第15届国际光合作用大会)

会议地点: 北京

会议语种:英文

页码: 231-233

在线出版日期: 2010-08-22（万方平台首次上网日期，不代表论文的发表时间）